Advanced Methods for Accessing Protein Shape-Shifting Present New Therapeutic Opportunities.
Trends Biochem Sci. 2018 Dec 13;:
Authors: Knoverek CR, Amarasinghe GK, Bowman GR
A protein is a dynamic shape-shifter whose function is determined by the set of structures it adopts. Unfortunately, atomically detailed structures are only available for a few conformations of any given protein, and these structures have limited explanatory and predictive power. Here, we provide a brief historical perspective on protein dynamics and introduce recent advances in computational and experimental methods that are providing unprecedented access to protein shape-shifting. Next, we focus on how these tools are revealing the mechanism of allosteric communication and features like cryptic pockets; both of which present new therapeutic opportunities. A major theme is the importance of considering the relative probabilities of different structures and the control one can exert over protein function by modulating this balance.
PMID: 30555007 [PubMed – as supplied by publisher]